IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Fractors (BDF) and histones interaction in Trypanosoma cruzi.
Autor/es:
VANINA VILLANOVA; ESTEBAN SERRA
Lugar:
Rosario
Reunión:
Congreso; XLII Reunion Anual de la Sociedad Argentina de Investigación Bioquímica; 2006
Institución organizadora:
SAIB
Resumen:
Chromatin structure regulation can be accomplished by at least two mechanisms: ATP-dependent chromatin remodeling and covalent histone modifications. Several chromatin remodeling factors and HAT complexes contain at least one bromodomain motif. This motif binds acetyl-lysine and it is present in many transcriptional regulators, participating in chromatin structure remodeling and transcription control. Little is known about chromatin structure and transcriptional regulation in trypanosomatids. Recently, chromatin modification has been described along T. cruzi life cycle and epimastigotes cell cycle. Coding sequences for putative methytransferases, HAT, HDAC and BDF have been revealed after the genome project. So, an epigenetic gene control regulation is suggested. We are studying BDF functions in T. cruzi transcription and chromatin structure regulation. TcBDF1, 2 and 3 were studed in T. cruzi epimastigotes. TcBDF2 was detected by WB in nuclear extracts and its localization was confirmed by IFI. However we were not able to detect TcBDF1 and 3. To find TcBDF2 in vivo interactors, we used TAP-Tag system. Protein complexes were recovered and eluted proteins were evidenced by SDS-PAGE. In parallel they were found to interact with T. cruzi histones by Far WB, using histones purified by acid extraction and recombinant TcBDFs. Different interaction patterns have been shown for each TcBDF.