IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Draft Genome Sequence of Enterococcus mundtii CRL1656
Autor/es:
ESPARIZ M, RAYA R, SESMA F, REPIZO G, BLANCATO V, MORTERA P, ALARCÓN S, MAGNI C.
Lugar:
Potrero de los Funes
Reunión:
Congreso; XLV Reunión Anual de SAIB; 2011
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Enterococcus mundtii is a coccoid, Gram-positive, pigmented bacterium that belongs to the suborder Enterococcaceae. E. mundtii strain CRL1656 was isolated from raw cow milk from the region of Trancas (Tucumán, Argentina). This bacteriocinogenic lactic acid bacterium has been proposed as a probiotic microorganism to prevent mastitis in cows. We present the draft genomic sequence of this strain which was assembled in 450 contigs. The sequence was obtained using a whole-genome shotgun strategy (231,679 reads totaling ~89 Mb; ~29-fold coverage) with a 454 GS Titanium pyrosequencer. The genome was 3,102,251 bases in length, with a mean GC content of 38.31%. A total of 2,764 coding sequences (CDS) and 65 structural RNAs (49 tRNAs) were predicted. Annotation covered 297 RAST subsystems (43%) with 1,176 CDS of which 64 CDS were labeled as hypothetical proteins. As this strain is adapted to the dairy and plant environment a large set of genes related to sugar metabolism was detected (406 genes). We also found 22 genes that might be related to resistance to antibiotics and toxic compounds. Noteworthy, 3 of them encoded putative b-lactamases, 4 resistance to fluoroquinolones and 2 multidrug efflux pumps. A cluster of 3 genes involved in bacteriocin synthesis was also found. Further comparative and experimental work will be needed to determine the probiotic characteristics of this microorganism.