IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of C/EBP and Sp1 in the regulation of CKa and CCTa expression during neuronal differentiation.
Autor/es:
DOMIZI, P AND BANCHIO C
Reunión:
Congreso; SAIB; 2010
Resumen:
Neuronal differentiation is characterized by neuritogenesis and neurite outgrowth, processes which are dependent on membrane biosynthesis. We previously demonstrated that the production of phosphatidylcholine (PC), the major membrane phospholipid, is stimulated during RA-induced neuronal differentiation in Neuro-2a cells. PC synthesis is promoted by an ordered and sequential activation of choline kinase alpha (CKa) and choline cytidylyltransferase alpha (CCTa). Early after RA stimulation, the increase in PC synthesis is mainly governed by the biochemical activation of CCTa, later, the transcription of CKa- and CCTa-encoding genes is induced. To elucidate the mechanism by which the expression of CKa and CCTa is induced during RA-induced neuritogenesis, we used promoter reporter assays and EMSAs. These analyses allowed us to postulate that C/EBP induces CKa expression, and Sp1 is involved in the regulation of the basal CCTa expression and in its induction during RA-induced neuritogenesis.