ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ESSENTIAL OILS AND NON VOLATILE POLYPHENOLS FROM TWO PHENOTYPES OF Rosmarinus officinalis L.

MAURO GAYA, CATALINA VAN BAREN, MIGUEL ELECHOSA, MARÍA V BARNI, MIGUEL JUÁREZ, ADRIANA OJEDA, SILVIA MORENO

Salón Auditórium de la subsede CEPROCOR. Valle de Punilla, Córdoba

Jornada; II -- Reunión de Biotecnología Aplicada a Plantas Medicinales y Aromáticas; 2009

REDBIO Argentina Asociación Civil Y MINISTERIO DE CIENCIA Y TECNOLOGIA DE CORDOBA

Essential oils and non-volatile polyhenol extracts are of interest due to their antioxidant and antimicrobial properties. In this work the content and composition of bioactives are compared in both fractions isolated from two phenotypes of Rosmarinus officinalis L. grown under the same soil and climate conditions. We investigated their potential relationship between the composition and the antioxidant and antimicrobial activities. The essentials oils were isolated by hydrodistillation and the ethanolic extract was obtained from leaves by solid/liquid extraction. Patterns of bioactives were analyzed by GC/MS and HPLC, respectively. The antioxidant activity was determined by the DPPH assay and the antimicrobial activity by the broth dilution method. Results showed that the chromatographic patterns of the non volatile polyhenols in both plants are qualitatively similar, although they differ significantly in the content of each main bioactive compound. The phenotype exhibiting the major content of non volatile diterpens resulted to posses the main antioxidant and antimicrobial activities. The volatile fraction of this plant also presented the highest antioxidant activity. In conclusion, both phenotypes of rosemary presented a typical pattern of bioactive molecules associated with a specific antioxidant and microbicide action.