PHYTOPOLYPHENOLS OF ROSEMARY: ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF VOLATILE AND NON VOLATILE FRACTIONS

GAYA M, VAN BAREN C, ELECHOSA M, BARNI MV, JUAREZ M, OJEDA A, MORENO S

Ciudad deTucumán

Congreso; XLV Reunión Anual SAIB,; 2009

Sociedad Argentina de Investigación Bioquímica

In this work the content and composition of polyphenols present in volatile and non volatile fractions isolated from two phenotypes of Rosmarinus officinalis L. grown under the same soil and climate are compared. The relationship between the composition and the antioxidant and antimicrobial activities were studied. Patterns of bioactives were analyzed by GC/MS and HPLC, the antioxidant activity was studied by the DPPH assay and the antimicrobial activity by the broth dilution method. Results showed that the chromatographic patterns of the non volatile polyhenols in both plants are qualitatively similar, although they differ significantly in the content of each main bioactive compound. The phenotype exhibiting the major content of non volatile diterpens resulted to posses the main antioxidant and antimicrobial activities. The volatile fraction of this plant also presented the highest antioxidant activity. In conclusion, both phenotypes of rosemary presented a typical pattern of bioactive molecules associated with a specific antioxidant and microbicide action.