CRYSTAL STRUCTURE AND EXPRESSION OF A PUTATIVE PHAGE-LIKE PROTEIN CODED IN THE GENOME OF A MARINE ANTARCTIC BACTERIA

PELLIZA L.; ARAN M; KLINKE S.; RINALDI J.; SYCZ G.; SMAL C; PARIS G.; GOLDBAUM F.; CICERO DO; LOPEZ JL; GUIMARAES B; LANZAROTI E; TURJANSKI A; VAZQUEZ S.; MAC CORMACK W

Congreso; 6th International Conference on Polar and Alpine Microbiology; 2015

Bizionia argentinensis JUB59T (B.a) was isolated from surface marine water in Potter Cove, (62° 149´ S; 58° 409´W), Antarctica and described as a new species. JUB59 is a psychrotolerant marine bacteria; Gram-negative, nonmotile rods forming yellowish orange colonies on marine agar (Bercovich et al. 2008).The sequencing of JUB59 genome (GenBank AFXZ00000000) suggested interesting features such as UV resistance, hydrolytic exoenzymes, and nitrogen metabolism. Several putative gene sequences, of unknown biological function and with possible new folds were selected, cloned, expresed and its products were purified from the culture broth. One of the B.a gene products, named C24, showed structural homology with a T4 phage tail fiber protein. In this work, we investigate the structure and expresion of C24.