IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Development of a quantitative immunoassay for gluten - free food analysis based on a new antigen-binding strategy.
Autor/es:
VANINA DOÑA1, MARIELA URRUTIA2, FERNANDO GOLDBAUM2 AND FERNANDO G. CHIRDO1.
Lugar:
Amsterdam, the Netherlands
Reunión:
Congreso; 13th International Coeliac Disease Symposium 2009; 2009
Institución organizadora:
Congreso Mundial de Enfermedad celiaca
Resumen:
Gluten-Free Diet is the most effective therapy for coeliac disease. To this end, gluten-free products must be analysed using high detectability quantitative tests. To increase protein recovery from food samples and to avoid false negative results, 60% ethanol extraction can be performed in the presence of denaturing agents. However, these agents can interfere with antigen recognition. In addition to conventional antibodies, camelids have heavy chain antibodies, in which the antigen binding site is formed by only one compact domain (called VHH) and are more resistant to aggressive conditions. The aim of this work was to generate a new analytical tool for gliadin determination based on stable single domain antibody fragments (VHH).