IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Biochemical and behavioral parameters after eclosion in Ceratitis and comparison with Drosophila
Autor/es:
BOCHICCHIO, P; BODIN, H; PÉREZ, M.; RABOSSI, A; QUESADA-ALLUÉ
Lugar:
Rosario
Reunión:
Congreso; L Reunión Anual-Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2014
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)
Resumen:
Despite numerous studies on the life cycle of Cychlorraphan flies and other Dipterans, many aspects related to holometaboulous metamorphosis are poorly known. Several details on the emergence of the immature adult remain unknown, both in the Ceratitis capitata and in Drosophila. We focused our interest in the extrication behavior of both flies, and in the subsequent body maturation. Our aim was to correlate behavioral performance with metabolic parameters and with the sclerotization machinery. To analyze in detail a significant number of flies and parameters, different arenas and a novel imaging recording system was set up. An image and movement analytical software was de novo implemented. Data on the timing of events during extrication of both flies indicated that the respective programs are very similar, although emergence is differently timed. However, from just exarate adults to fully body maturation, the body maturation phase, significant differences were detected. The timing was normalized to understand the equivalent developmental times. Rapid recovery of glycogen and lipid reserves occurs immediately after emergence. Variation of the sclerotization/tanning machinery (N-B-alanyldopamine synthesis) was also analyzed. Other events were recorded in C. capitata and for the first time the above behavioral parameters were related to wet/dry weight, water content and O2 consumption.