IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Crystal structures of BA41, BA42, and C24: three proteins of unknown function from the Antarctic bacterium Bizionia argentinensis (poster)
Autor/es:
LISANDRO OTERO; SEBASTIÁN KLINKE; MARÍA L. CERUTTI; MARTIN ARAN; CLARA SMAL; LEONARDO PELLIZA; DANIEL O. CICERO; FERNANDO A. GOLDBAUM
Lugar:
Mar del Plata
Reunión:
Congreso; X Reunión Anual de la Asociación Argentina de Cristalografía (AACr); 2014
Institución organizadora:
Asociación Argentina de Cristalografía (AACr)
Resumen:
A new bacterial species designated as Bizionia argentinensis (BA) was isolated from the Antarctic sea surface [1]. The genome of BA was recently decoded [2] and constitutes a relevant source for the discovery of new proteins showing biological activity in extreme conditions of low temperatures. In order to identify suitable targets for structure determination among the proteins codified in the BA genome, a protocol based on bioinformatics analysis and NMR screening was set up. By this method we were able to select proteins that given their solubility and folding became good candidates for structural determinations. Here, we show the crystal structures of three of them, the proteins BA41, BA42, and C24. By comparing these protein structures against those deposited in the Protein Data Bank (PDB) we found that BA41 displays a fold similar to a phosphatase (Fig. 1A), BA42 is the first structure of a member of the PF04536 family comprised of a stand-alone TPM domain (Fig. 1B) [3], and C24 presents an overall shape that resembles the bacteriophage T4 long tail fiber receptor-binding tip (Fig. 1C). Our structural and biochemical studies will allow us to discuss their function and potential roles.  [1] A. Bercovich, S.C. Vazquez, P. Yankilevich, S.H. Coria, M. Foti, E. Hernández, A. Vidal, L. Ruberto, C. Melo, S. Marenssi, M. Criscuolo, M. Memoli, M. Arguelles, W.P. Mac Cormack, Int. J. Syst. Evol. Microbiol., 58 (2008) 2363?2367.[2] E. Lanzarotti, L. Pellizza, A. Bercovich, M. Foti, S.H. Coria, S.C. Vazquez, L. Ruberto, E. Hernández, R. Dias, W.P. Mac Cormack, D.O. Cicero, C. Smal, M. Nicolas, A. Ribeiro Vasconcelos, M. Marti, A. Turjanski,  J. Bacteriol., 193 (2011) 6797?6798.[3] M. Aran, C. Smal, L. Pellizza, M. Gallo, L.H. Otero, S. Klinke, F.A. Goldbaum, E.R. Ithurralde, A. Bercovich, W.P. Mac Cormack, A.G. Turjanski, D.O. Cicero, Proteins (2014) In Press.