IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Astrocyte Heterogeneity Derived from a Single Spinal Cord Progenitor Domain
Autor/es:
MARÍA MICAELA SARTORETTI; ALEJANDRA ABDALA; GUILLERMO LANUZA
Lugar:
Huerta Grande, Córdoba
Reunión:
Congreso; XXIX Reunión anual de la Sociedad Argentina de Neurociencias; 2014
Institución organizadora:
Sociedad Argentina de Neurociencias
Resumen:
Astrocytes are highly heterogeneous in their morphology and specification. An interesting question is to unravel the origin of such diversity. By performing genetic fate mappings of a defined subset of progenitors, termed p0, in the neural tube, we identified that it generates astrocytes that follow a stereotyped migration pathway. We named them as "vA0" (ventral astrocytes from p0 progenitor domain). Their differentiation starts at stage E14.5 in the mouse embryo and follow radial migration from the ventricular zone to the white matter. In the postnatal spinal cord, the location of vA0 is exclusively restricted to a macrodomain at the sulcus limitans. To assess vA0 cell morphologies, we generated fluorescent mosaic embryos. We found that during development, vA0 cells exhibit two types of morphologies which are closely associated with their positioning in the spinal cord: 1) without processes and 2) displaying a radial shape with cellular processes toward the ventricle and the pia mater. At neonatal stage, vA0 precursors generate both fibrous (radial morphology, mostly on the white matter, contacting the pia) and protoplasmic astrocytes (star-shaped, located on the grey matter). Furthermore, a small proportion of vA0 still retain a radial morphology with processes contacting thepia. In summary, we found that a restricted set of progenitors gives rise to astrocytes that occupy a defined region of the CNS but display morphological heterogeneity in the postnatal spinal cord.