Toxicological analysis of rosemary bioactives with potential use in clinical applications against bacteria-resistant in rabbits

OJEDA-SANA AM; CÁCERES GUIDO PA ; ASPREA M; VAN BAREN C; BALBARREY Z; MACCHI A ; FILIPPO D; MORENO S

La habana

Congreso; LATINFARMA: 20 Congreso Latinoamericano de Farmacología y Terapéutica. 5to Congreso Iberoamericano de Farmacología. 5to Congreso Internacional y 11 Congreso Nacional de la Sociedad Cubana de Farmacología; 2013

Sociedad Cubana de Farmacología

This work documented the plant drug exposure of rosemary bioactives in the skin of rabbits. Previously, we demonstrated the pharmacological potential of several rosemary compounds in vitro and in two skin infection models in mice against human pathogenic bacteria1-4. However, remains to investigate if they have no toxicological effects on the skin. The aim of this study was to evaluate induction of irritation and/or dermal corrosion, and inflammatory reactions by contact with the skin of the rosemary bioactive in rabbit. The protocol included a three topical dose at 0 hs, 24 hs and at 48 hs of starting the treatment. The toxicity was assessed by determination of erythema at 1 h, 24 hs, 48 hs and 72 hs of treatment on four skin areas of 4 cm2 on each animal by photographic analysis. The edema was investigated by ultrasound analysis of the treated area. The animals were euthanized after 72 hs and histopathological analyzes of the skin treated with the topical cream containing the bioactive or vehicles were performed in biopsy specimens stained with hematoxylin eosin. Also, the content of plant compounds was determined in the serum of the animals by gas chromatography after 30 min, 1 hour or 24 h of treatment. Results showed that the skin treated with the plant compounds no showed significant signs of swelling after 4 days of treatment and three consecutive doses. Similarly, epidermal layer scanned by ultrasound showed the same dimension in the group treated with the active plant and the group treated with the vehicle. This work has a direct impact on areas of health, since it is important to developed new green alternatives for the treatment of bacterial resistance. 1Free Rad Res 2006, 40:223; 2BLACPMA 2009, 8 (3):219; 2Pediatric Res 2012, 72(1):109; 3Food Control 2013, 31:189.