IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Interaction between biofilm matrix components in Rhizobium leguminosarum
Autor/es:
ABDIAN PL; CARAMELO J; VOZZA NF; VON BILDERLING C; RUSSO DM; PIETRASANTA LI; ZORREGUIETA A
Lugar:
Puerto Varas
Reunión:
Congreso; XII Congreso PABMB, XLIX Reunión Anual de la Soc.Argentina de Investigación Bioqca y Biología Molecular (SAIB), XXXVI Reunión de la Soc. de Bioqca y Biología Molecular de Chile, LVI Reunión anual Soc. Biología de Chile, IV Reunión Soc.Latinoam. Proteinas; 2013
Institución organizadora:
PABMB, Soc.Argentina de Investigación Bioqca y Biología Molecular (SAIB), Soc. de Bioqca y Biología Molecular de Chile, Soc. Biología de Chile, Soc.Latinoam. Proteínas
Resumen:
INTERACTION BETWEEN BIOFILM MATRIX COMPONENTS IN Rhizobium leguminosarum Abdian, P1.,Caramelo, J2.,Vozza, N1.,Von Bilderling, C3.,Russo, D1.,Pietrasanta, L3.,Zorreguieta, A1.,1Laboratorio de Microbiología Molecular y Celular, Fundación Instituto Leloir.2Laboratorio de Biología Estructural y Celular, Fundación Instituto Leloir.3Centro de Microscopías Avanzadas, Universidad de Buenos Aires - Argentina . Rhizobia are soil bacteria that infect root tissue and induce the development of nodules on leguminous plants under conditions of nitrogen starvation. The ability of rhizobia to grow in varied environmental conditions is probably due to its ability to produce different types of biofilm. The main component of the extracellular matrix of the biofilm developed on a glass surface is an acidic exopolysaccharide or EPS that provides support to the biofilm structure. Proteins secreted by a type I secretion system (PrsDE) have been shown to be important for biofilm development. Interestingly, some of them possess one or more characteristic Ra/CHDL (cadherin like-) domains that confer carbohydrate binding ability. Among them, we have characterized RapA2, a unipolar calcium binding lectin that specifically recognizes the EPS and the glycanases Ply that regulate the length of EPS molecules in the extracellular medium. A characteristic distribution of EPS molecules length is important for the development of an ordered polysaccharide network. Thus, Ply mutants are unable to form a typical biofilm. On the other hand, anomalous levels of RapA2 affect the production of surface acidic polysaccharides. A model depicting the interaction of RapA2, Ply glycanases and acidic polysaccharides during the progress of biofilm matrix development is presented. Supported by CONICET and Agencia de Promoción Científica y Tecnológica-FONCYT