Exarate adult characteristics and imago functional senescence in the medfly Ceratitis capitata

BOCHICCHIO, PABLO A.; PUJOL LEREIS, LUCIANA M.; RABOSSI, ALEJANDRO; QUESADA ALLUÉ, LUIS A.

Buenos Aires

Congreso; SAIB 2013; 2013

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

Senescence, behavioral and biochemical parameters were analyzed during the life cycle of the Medfly. Average survival was 36.1±1.2 days, whereas maximum longevity was 81.7±2.1 days. On the basis of the unusual first supine behavior (FSP), at day 10, proposed as a predictor of death, we arbitrarily defined the span of youth stage. This young imago period was defined according to the absence of significant death (since exarate adult emergence until day 20). A reduction in the locomotor activity since FSP, during the transition from early to late young stages, was not accompanied by changes in energetic resources (Glycogen and Lipids). However, there was an increase of peroxidated brain lipids (TBARS), starting early during the youth period, whereas the antioxidant capacity decreased until day 30. Nevertheless TBARS only increased in thoracic muscles at day 30, in correlation with the reduction in locomotor activity and survival. These results agree with the functional senescence index, derived from neutral and polar lipid profiles (Pujol-Lereis et al., 2012). In addition to confirm that functional senescence parameters differ depending on the tissue/organ, our results also support the predominant idea about the role of ROS species in peroxidation. This was made evident by different stress-induced experiments.