IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Transcriptional Regulation of Late Neurogenic Events in the Neural Tube
Autor/es:
DANIELA DIBELLA; ABEL CARCAGNO; YANINA PETRACCA; MICAELA SARTORETTI; GUILLERMO LANUZA
Lugar:
Huerta grande
Reunión:
Congreso; XXVII Congreso anual de la Sociedad Argentina de Investigación en Neurociencias; 2012
Resumen:
This work focuses in understanding the genetic mechanisms that control the differentiation of neuronal cell types in the embryonic spinal cord. We are interested in a recently described population of spinal neurons, the CerebroSpinal Fluid contacting Neurons (CSF-cN), that originate in the ventral neural tube late during embryonic development. Genetic lineage tracing in vivo experiments and expression analysis in mice show that CSF-cN progenitors express both the transcription factors Ascl1 and Gata3. We show that Ascl1 is required for CSF-cN differentiation, as they are missing in Ascl1 KO mice. We temporally restricted Ascl1 expression using conditional KO mice, revealing that Ascl1 exerts its critical actions around the time of CSF-cN differentiation. Our results indicate that Gata3 plays a key role in CSF-cN development, as these neurons are severely affected in the absence of Gata3. We determined that Ascl1 precedes Gata3 expression and that Gata3 is absent in ventral progenitors from Ascl1 mutants. On the contrary, no change was found in Ascl1 expression in Gata3 absense. In summary, we identified two key and specific regulators of CSF-cN specification. Our results show that Ascl1 and Gata3 act sequentially in controlling late neurogenic events in the ventral neural tube.