IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
P H Y S I O L O G I C A L A N D M O L E C U L A R CHARACTERIZATION OF INTERACTIONS BETWEEN SHADEAVOIDANCEAND TEMPERATURE
Autor/es:
MAXIMILIANO SANCHEZ; CHRISTIAN DAMIAN LORENZO; SOFIA ARELLANO; PABLO D. CERDÁN
Lugar:
Mendoza
Reunión:
Congreso; Reunion anual de la Sociedad Argentina de Investigación Bioquímica; 2012
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica
Resumen:
Light signals are essential to the growth and development of plants. Blue light is sensed by three families of photoreceptors: cryptochromes (CRY1-2), phototropins (PHOT1-2) and LOV domain (ZTL, FKF, LKP2) photoreceptors, whereas red and far red light is perceived by the phytochromes (PHYA-E). The ability to withstand environmental temperature variation is essential for plant survival. It’s well know that shade avoidance responses that are sensed by the phytochromes, induce similar phenotypes to those induced by high temperature. Based on this, we decided to characterize the temperature dependence of the phyB mutant phenotype at the hypocotyl growth level, as a model of temperature and light interactions (Fig.1). To address to this aim we used physiological measures (hypocotyl length), transcriptomic (microarrays) and qRT-PCR. The results show that at normal temperature ranges, plants are permanently sensing ambient temperature, the responses being close to linear (Fig.2). Further, phyB and other photoreceptors play a key role buffering this response (Fig.1 and Fig.4). Consistent with this proposition, the gene expression analysis show higher levels of auxin-responsive genes only when the interaction between light and temperature signaling is evident (Fig.3 and Fig.5). In conclusion, some photoreceptors play an important role in buffering exacerbated responses to temperature.