IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A high-throughput screening for structural proteomics of psychrotolerant bacteria
Autor/es:
PELLIZZA L; ARAN M; SMAL C; GALLO M; LANZAROTTI E; TURJANSKY A; CICERO D
Lugar:
Mendoza
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Bioquímica y Biología Molecular; 2012
Institución organizadora:
SAIB
Resumen:
The genome of the bacterium Bizionia argentinensis sp. Nov. (BA), isolated in the National Antarctic Territory, was recently deciphered and is a starting point for the discovery of new proteins with biological activity at low temperatures. Structural genomics of BA will yield invaluable information about protein folding and function as well as the structural determinants responsible for the cold adaptation mechanisms. The present work describes the first high-throughput strategy for the screening of targets for structural proteomics of psychrotolerant bacteria. Using bioinformatic analysis we selected 50 genes coding for proteins of unknown function. After an initial solubility screening, 22 targets were chosen and characterized by Nuclear Magnetic Resonance (NMR) to select those proteins with suitable characteristics for three-dimensional structure determination. NMR spectroscopy and X-ray crystallography were used in parallel to increase the number of resolved structures. Here we show our preliminary experimental results.