IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Diversity of Burkholderia species in Argentinian soils with different agricultural management
Autor/es:
DRAGHI, W.O.; DEGROSSI, J.; ABDIAN, P.L.; SOLER BISTUÉ, A.J.; WALL, L.G.; ZORREGUIETA, A.
Lugar:
San Miguel de Tucumán
Reunión:
Congreso; VII Congreso Argentino de Microbiología General, SAMIGE del Bicentenario; 2011
Resumen:
In the context of the BIOSPAS global project, we performed a survey to recognize the
biodiversity of Burkholderia in soils with different agricultural management within the main
agricultural production area of Argentina. The Burkholderia genus is widely distributed in the
environment, including in some associations with plants, which could be beneficial to crop
production. Three sites (Bengolea, Monte Buey and Pergamino) and three treatments: natural
environment (AN), sustainable agricultural practices (BPA), and non- sustainable practices
(MPA), were chosen for soil sampling. Burkholderia was selected on PCAT medium, and
confirmed by recA amplification. Diversity was determined by both RFLP and sequencing of a
recA amplicon. We identified 7 different species (B. cepacia, B. ambifaria, B. gladioli, B.
cenocepacia, B. caribensis, B. caledonica, B. terricola). Besides, we also identified several
strains, which match strains described as uncultured and others to which no species could be
assigned. To analyze the effect of agricultural managements, the Shannon biodiversity index
was obtained at each location. In general, the index evolved until reach a stable value, showing
that sufficient samples were analyzed. We observed that in 2 out of 3 sites, AN showed a higher
diversity, according to the Shannon Index. We also observed an increasing proportion of B.
ambifaria from AN to BPA and MPA. This observation suggests that agricultural practices
decrease the incidence of some Burkholderia species. Comparation of these results with
metagenomic data obtained by other group within the BIOSPAS consortium will allow us to
determine if changes in the biodiversity profiles is exclusive of culturable species, or if it is a
general behavior of the Burkholderia pool in soils (culturable and non culturable species).