IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression of basi during barley germination and its relation with nitrogen content.
Autor/es:
CODÓ, P.C., PRYSTUPA, P., ZAVALA, J.A., WOLOSIUK, R.A. AND PAGANO, E.A.
Lugar:
San Luis
Reunión:
Congreso; XLVII Reunión Nacional de la SAIB; 2011
Institución organizadora:
Sociedad Argentina de Investigaciòn Bioquímica y Biología Molecular
Resumen:
Plant seeds have a large number of proteinaceous inhibitors which act on
a-amylase and proteases. Among Kunitz-type
inhibitors there is one of endogenous a-amylase in barley named
BASI (Barley a-Amylase Subtilisn Inhibitor). BASI is synthesized during
grain filling and is an abundant protein of the mature seed. Thioredoxin h (Trx h), a ubiquitous protein
containing a redoxactive disulfide group, reduce storage proteins and
inactivate various inhibitor proteins. The aim of this work is to determine the
response to nitrogen content in Trx h and BASI expression
during germination of barley. To this end, an experiment of nitrogen fertilization
was conducted in barley. The resulting seeds were germinated and were measured
both expression of Trx and BASI. The complete sequence of BASI was sequenced in
cDNA synthesized from RNA of 48-hour-germination seeds. It was confirmed that
the inhibitor is expressed in germinating seeds. Both Trx and inhibitor were
greater in seeds with high nitrogen content than in seeds with lower nitrogen
content. The understanding of these biochemical relationships will help to
understand the relation between grain composition and the mechanisms of starch degradation
during germination.