IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Analysis of transcriptional regulation of an adhesin in Brucella suis and Brucella abortus.
Autor/es:
AROCENA GM, RUIZ V, POSADAS DM, ZORREGUIETA A, SIEIRA R
Lugar:
Puerto Madryin
Reunión:
Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Species belonging to the Brucella genus are intracellular pathogens responsible of an endemic disease called brucellosis. Different Brucella species vary in their host preference. It has been shown that Brucella is able to adhere to epithelial cell lines However, bacterial factors involved in adhesion and/or invasion remain unexplored. Recently, by heterologous and mutational approaches, we found that several members of the Type-V Secretion System autotransporter families of Brucella suis participate in bacterial adhesion to abiotic surfaces and HeLa cells. Our goal is to identify signals and regulatory pathways that control transcription of these adhesins in B.  suisand Brucella abortus. Analyses of the adhesin genes and putative regulatory regions revealed differences in both promoter sequence and protein length between these species. By EMSA assays, we identified several regulators that interact specifically with the promoter region of one of the autotransporters . We have previously documented that these transcription factors also participate in the regulation of other important virulence factor of Brucella. By DNAase I footprinting, we identified their binding sites to the btaE promoter region. In order to determine the specific role of these regulators, we constructed transcriptional fusions to the lacZ gene and measured β-galactosidase activity in different mutant backgrounds.[u1]   Differences in adhesin activity and/or expression between Brucella species may contribute to a differential tissue tropism.