INTEMA   05428
INSTITUTO DE INVESTIGACIONES EN CIENCIA Y TECNOLOGIA DE MATERIALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Polylactic acid sheets seeded with genetically modified ovine diaphragmatic myoblasts for myocardial regeneration. Preliminary results.
Autor/es:
F.D. OLEA; R. DEWEY; A. ORLOWSKI; A. CROTTOGINI; C.S. GIMENEZ; M.R. BAUZA; G.A. ABRAHAM; L. CUNIBERTI; P. LOCATELLI; F. MONTINI BALLARIN; A. DE LORENZI
Lugar:
Mar del Plata
Reunión:
Encuentro; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI) y XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE); 2016
Institución organizadora:
Sociedad Argentina de Investigación Clínica (SAIC), Sociedad Argentina de Inmunología (SAI) y Sociedad Argentina de Farmacología Experimental (SAFE)
Resumen:
We tested the hypothesis that polylactic acid (PLA) sheets seeded with diaphragmatic myoblasts (DM) overexpressing connexin-43 (Cx43) would decrease infarct size and improve left ventricular (LV) function in sheep with chronic myocardial infarction (MI). Forty minutes after coronary artery ligation, PLA sheets without DM (PLA, control group, n=4) or PLA sheets with DM overexpressing Cx43 (PLA-DM-Cx43 group, n=4) were sutured covering the infarcted zone and 1 cm of the adjacent peri-infarct zone. Baseline echocardiograms confirmed that all animals displayed normal LV function prior to infarction. Cardiac magnetic resonance with gadolinium enhancement was performed at 3 and 45 days after MI to determine infarct size and LV function early and late after treatment, respectively. Blinded data analysis revealed that infarct size expressed as ml of infarcted tissue decreased in the PLA-DM-Cx43 group from 8.0±1.83 ml at day 3 to 6.25 ±2.2 ml at day 45 (p